Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy

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McConnell, G. (2005) Noise analysis of a white-light supercontinuum light source for multiple wavelength confocal laser scanning fluorescence microscopy. Journal of Physics D: Applied Physics, 38 (15). pp. 2620-2624. ISSN 1361-6463 (http://dx.doi.org/10.1088/0022-3727/38/15/013)

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Abstract

Intensity correlations of a Ti : sapphire, Kr/Ar and a white-light supercontinuum were performed to quantify the typical signal amplitude fluctuations and hence ascertain the comparative output stability of the white-light supercontinuum source for confocal laser scanning microscopy (CLSM). Intensity correlations across a two-pixel sample (n = 1000) of up to 98%, 95% and 94% were measured for the Ti : sapphire, Kr/Ar and white-light supercontinuum source, respectively. The white-light supercontinuum noise level is therefore acceptable for CLSM, with the added advantage of wider wavelength flexibility over traditional CLSM excitation sources. The relatively low-noise white-light supercontinuum was then used to perform multiple wavelength sequential CLSM of guinea pig detrusor to confirm the reliability of the system and to demonstrate system flexibility.

ORCID iDs

McConnell, G. ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
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