Neuroprotection by a selective oestrogen receptor {beta} agonist in a mouse model of global ischaemia

Carswell, Hilary and Macrae, I.M. and Gallagher, L. and Harrop, E. and Horsburgh, K.J. (2004) Neuroprotection by a selective oestrogen receptor {beta} agonist in a mouse model of global ischaemia. American Journal of Physiology - Heart and Circulatory Physiology, 287 (4). H1501-H1504. IDS Number: 854IH. ISSN 0363-6135

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Official URL: https://doi.org/10.1152/ajpheart.00227.2004

Abstract

The present study employs selective estrogen receptor (ER) agonists to determine whether 17-estradiol-induced neuroprotection in global ischemia is receptor mediated and, if so, which subtype of receptor (ERα or ER) is predominantly responsible. Halothane-anesthetized female C57Bl/6J mice were ovariectomized, and osmotic minipumps containing ER agonist diarylpropiolnitrile (DPN) (8 mg·kg–1·day–1, n = 12) or vehicle (50% DMSO in 0.9% saline) (n = 9) or ERα agonist propyl pyrazole triol (PPT) (2 mg·kg–1·day–1, n = 13) or vehicle (50% DMSO in 0.9% saline) (n = 10) were implanted subcutaneously. One week later transient global ischemia was induced by bilateral carotid artery occlusion under halothane anesthesia, and the mice were perfusion fixed 72 h later. ER agonist DPN significantly reduced ischemic damage by 70% in the caudate nucleus and 55% in the CA1 region compared with vehicle controls (P < 0.05, Mann-Whitney U-statistic). In contrast, pretreatment with the ERα agonist PPT had no effect on the extent of neuronal damage compared with controls. The data indicate a significant estrogen receptor-mediated neuroprotection in a global cerebral ischemia model involving ER.

Creators(s):	Carswell, Hilary ORCID: https://orcid.org/0000-0002-0938-1212, Macrae, I.M., Gallagher, L., Harrop, E. and Horsburgh, K.J.;
Item type:	Article
ID code:	10979
Keywords:	neuroprotector, oestrogen receptor , mouse model, global ischaemia, Pharmacy and materia medica, Physiology, Cardiology and Cardiovascular Medicine, Physiology (medical)
Subjects:	Medicine > Pharmacy and materia medica
Department:	Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user:	Strathprints Administrator
Date deposited:	08 Nov 2011 14:26
Last modified:	02 Oct 2020 04:09
Related URLs:	Publisher
URI:	https://strathprints.strath.ac.uk/id/eprint/10979
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