Chiral analysis of methylphenidate and dextromoramide by capillary electrophoresis

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Denk, Oliver M. and Watson, D.G. and Skellern, G.G. (2001) Chiral analysis of methylphenidate and dextromoramide by capillary electrophoresis. Journal of chromatography. B, Biomedical sciences and applications, 761 (1). pp. 61-68. ISSN 1387-2273 (https://doi.org/10.1016/S0378-4347(01)00300-0)

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Abstract

Capillary electrophoretic methods have been developed to separate the enantiomers of methylphenidate (MPH) and dextromoramide. For MPH separation was achieved with heptakis (2,6-di-O-methyl)-beta -cyclodextrin (DMCD) as chiral selector in a 100 mM phosphoric acid buffer adjusted to pH 3.0 with triethanolamine. Commercial samples Of D,L-erytho-MPH HCl and D,L-threo-MPH HCl were analysed using the method. There was no evidence of the presence of D,L-threo-MPH HCl in D,L-etytho-MPH HCl and vice versa. The ratio of the enantiomers was determined for each diasteroisomer. Hydroxypropyl-beta -cyclodextrin was the chiral selector of choice for the chiral separation of the enantiomers of moramide. The separation which gave a resolution of about 3.5 was achieved in 4 min using only a 6 cm of length of capillary. In a sample of dextro-R-moramide tartrate only a small quantity (4.9% w/w) of levo-S-moramide was detected with this method.

ORCID iDs

Denk, Oliver M., Watson, D.G. ORCID logoORCID: https://orcid.org/0000-0003-1094-7604 and Skellern, G.G.;
CORE (COnnecting REpositories)