Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells

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Dever, G. and Stewart, L.J. and Pitt, A.R. and Spickett, C.M. (2003) Phospholipid chlorohydrins cause ATP depletion and toxicity in human myeloid cells. FEBS Letters, 540 (1-3). pp. 245-250. ISSN 0014-5793 (https://doi.org/10.1016/S0014-5793(03)00271-0)

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Abstract

Chlorohydrins of stearoyl-oleoyl phosphatidylcholine (SOPC), stearoyl-linoleoyl phosphatidylcholine, and stearoyl-arachidonyl phosphatidylcholine were incubated with cultured myeloid cells (111,60) for 24 h, and the cellular ATP level was measured using a bioluminescent assay. The chlorohydrins caused significant depletion of cellular ATP in the range 10100 muM. The ATP depletion by the phospholipid chlorohydrins was slightly less than that of 4-hydroxy-2-nonenal, but greater than that of hexanal, trans-2-nonenal, and autoxidised palmitoyl-arachidonoyl phosphatidylcholine. SOPC chlorohydrin was also found to cause loss of viability in U937 cells, and thus phospholipid chlorohydrins could contribute to the formation of a necrotic core in advanced atherosclerotic lesions.

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