Brown, R. and Smith, W.E. and Graham, D. (2001) Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces. Tetrahedron Letters, 42 (11). pp. 2197-2200. ISSN 0040-4039Full text not available in this repository. (Request a copy from the Strathclyde author)
A method for the addition of a benzotriazole moiety to the 5'-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.
|Keywords:||HPLC, nucleic acids, phosphoramidites, solid-phase synthesis, SERS, Chemistry, Biochemistry, Organic Chemistry, Drug Discovery|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry
|Depositing user:||Mr Derek Boyle|
|Date Deposited:||05 May 2006|
|Last modified:||06 Jan 2017 03:09|