Picture of a sphere with binary code

Making Strathclyde research discoverable to the world...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. It exposes Strathclyde's world leading Open Access research to many of the world's leading resource discovery tools, and from there onto the screens of researchers around the world.

Explore Strathclyde Open Access research content

Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition

Graham, D. and Grondin, A. and McHugh, C. and Fruk, L. and Smith, W.E. (2002) Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition. Tetrahedron Letters, 43 (27). pp. 4785-4788. ISSN 0040-4039

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.