Graham, D. and Grondin, A. and McHugh, C. and Fruk, L. and Smith, W.E. (2002) Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition. Tetrahedron Letters, 43 (27). pp. 4785-4788. ISSN 0040-4039
Full text not available in this repository. (Request a copy from the Strathclyde author)Official URL: http://dx.doi.org/10.1016/S0040-4039(02)00930-9
Abstract
A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.
| Item type: | Article |
|---|---|
| ID code: | 1064 |
| Keywords: | dna, oligonucleotide probes, cycloaddition, Chemistry |
| Subjects: | Science > Chemistry |
| Department: | Faculty of Science > Pure and Applied Chemistry |
| Related URLs: | |
| Depositing user: | Mr Derek Boyle |
| Date Deposited: | 16 May 2006 |
| Last modified: | 12 Mar 2012 10:36 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/1064 |
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