Graham, D. and Grondin, A. and McHugh, C. and Fruk, L. and Smith, W.E. (2002) Internal labeling of oligonucleotide probes by Diels-Alder cycloaddition. Tetrahedron Letters, 43 (27). pp. 4785-4788. ISSN 0040-4039Full text not available in this repository. (Request a copy from the Strathclyde author)
A new method of adding fluorescent labels to the middle of oligonucleotides is reported. Diets-Alder cycloaddition was used to add five fluorescent maleimides to an oligonucleotide containing a 2'-deoxyuridine modified at the 5-position with a spaced furan. This is a new approach to internal oligonucleotide chemistry that opens Lip a large range of possibilities for further conjugation.
|Keywords:||dna, oligonucleotide probes, cycloaddition, Chemistry, Biochemistry, Organic Chemistry, Drug Discovery|
|Subjects:||Science > Chemistry|
|Department:||Faculty of Science > Pure and Applied Chemistry|
|Depositing user:||Mr Derek Boyle|
|Date Deposited:||16 May 2006|
|Last modified:||29 Apr 2016 07:29|