Stevenson, D. and Morgan, C. and McLellan, L.A. and Grant, M.H. (2007) Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures. Toxicology, 231 (2-3). pp. 100-101. ISSN 0300-483X
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.
| Item type: | Article |
|---|---|
| ID code: | 9905 |
| Notes: | See also journal article: http://strathprints.strath.ac.uk/28452/ |
| Keywords: | cryopreserved hepatocyte monolayers, reduced glutathione, glutathione synthesis, expression of glutathione synthetase, Therapeutics. Pharmacology |
| Subjects: | Medicine > Therapeutics. Pharmacology |
| Department: | Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Faculty of Engineering > Bioengineering Unknown Department |
| Related URLs: | |
| Depositing user: | Strathprints Administrator |
| Date Deposited: | 04 Oct 2010 18:19 |
| Last modified: | 12 Mar 2012 10:52 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/9905 |
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