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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs. Strathprints provides access to thousands of Open Access research papers by University of Strathclyde researchers, including those from the School of Psychological Sciences & Health - but also papers by researchers based within the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

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Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

Stevenson, D. and Morgan, C. and McLellan, L.A. and Grant, M.H. (2007) Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures. Toxicology, 231 (2-3). pp. 100-101. ISSN 0300-483X

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Abstract

Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.