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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

Stevenson, D. and Morgan, C. and McLellan, L.A. and Grant, M.H. (2007) Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures. Toxicology, 231 (2-3). pp. 100-101. ISSN 0300-483X

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Abstract

Cryopreservation of hepatocytes as monolayers circumvents the problems of low post-thaw recovery and attachment in culture obtained with hepatocytes frozen in suspension. We have shown that cytochrome P450 dependent generation of reactive metabolites takes place readily in these cells (Stevenson et al., 2004), and in the present study the reduced glutathione (GSH) levels and the expression of the catalytic subunit of glutamate cysteine ligase (GCL) and of glutathione synthetase (GSS) have been investigated.