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Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures

Stevenson, D.J. and Morgan, C. and McLellan, L.I. and Grant, M.H. (2007) Reduced glutathione levels and expression of the enzymes of glutathione synthesis in cryopreserved hepatocyte monolayer cultures. Toxicology in Vitro, 21 (3). pp. 527-532. ISSN 0887-2333

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Abstract

Cryopreservation of monolayers of hepatocytes in a freezing medium containing 10% (v/v) dimethylsulfoxide, 90% (v/v) foetal calf serum retains cell morphology and viability, but cells lose up to 50% of their intracellular reduced glutathione. This is accompanied by a small increase in glutamate cysteine ligase expression in cryopreserved cultures, but glutathione synthetase expression is undetectable post-cryopreservation. Inclusion of ascorbic acid and alpha-tocopherol in the freezing medium improves maintenance of reduced glutathione content post-cryopreservation at 84% of the levels in non-cryopreserved monolayer cultures, but does not restore glutathione synthetase expression. The inability to synthesise reduced glutathione will mean that cryopreserved hepatocyte monolayers are more susceptible to toxic insults.

Item type: Article
ID code: 28452
Notes: See also abstract: http://strathprints.strath.ac.uk/9905/
Keywords: cryopreserved hepatocyte monolayers, Reduced glutathione, glutathione synthesis, expression of glutathione synthetase, Bioengineering, Engineering (General). Civil engineering (General), Toxicology
Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Technology > Engineering (General). Civil engineering (General)
Department: Faculty of Engineering > Bioengineering
Related URLs:
Depositing user: Ms Ashley Urie
Date Deposited: 28 Oct 2010 11:25
Last modified: 05 Sep 2014 04:51
URI: http://strathprints.strath.ac.uk/id/eprint/28452

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