Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Simultaneous analysis of basic, acidic and neutral compounds on an endcapped octadecylsilane silica-based monolith by pressure-assisted capillary electrochromatography

Zhang, Tong and Khadra, Ibrahim and Euerby, Melvin R. and Skellern, G.G. and Watson, David G. and Tettey, J.N.A. (2008) Simultaneous analysis of basic, acidic and neutral compounds on an endcapped octadecylsilane silica-based monolith by pressure-assisted capillary electrochromatography. Electrophoresis, 29 (4). pp. 944-951. ISSN 0173-0835

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

This simultaneous separation of basic, acidic and neutral analytes by pressure-assisted CEC (pCEC) using a hybrid (tetramethoxysilane and methyltrimethoxysilane) silica-based monolith, chemically modified with octadecyldimethylchlorosilane followed by endcapping with hexamethyldisilazane is described. The endcapping resulted in near Gaussian peaks for highly basic analytes such as nortriptyline without a significant loss in the EOF. The migration behaviour of analytes on this phase could be rationalised based on hydrophobicity, electrophoretic mobility and ion-exchange interactions. The high porosity of the monolith allowed manipulation of the linear velocity of mobile phases by the addition of varying amounts of pressure at the inlet to reduce analysis times and overcome the reversed migration of anionic species towards the detection window in cathodic EOF mode. The concomitant programmed application of pressure (2-4 bar) and voltage (27 kV) facilitated the simultaneous separation of four cationic, four neutral and two anionic compounds in 6 min with efficiencies ranging from 41 000 to 94 000, 57 000 to 77 000 and 180 000 to 210 000 theoretical plates/metre, respectively. The % RSD values of migration times and efficiencies in pCEC mode were less than 3.6 and 7.9%, respectively (n = 5).

Item type: Article
ID code: 7603
Keywords: capillary electrochromatography, endcapping, silica monoliths, pharmacology, biomedical sciences, Pharmacy and materia medica, Biochemistry, Clinical Biochemistry
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 25 Mar 2009 10:34
    Last modified: 04 Sep 2014 18:32
    URI: http://strathprints.strath.ac.uk/id/eprint/7603

    Actions (login required)

    View Item