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Confocal laser scanning microscopy (CLSM) study of hepatocytes cultured on collagen films and gels

Kataropoulou, M. and Goldie, E.I. and Connel, G. and Grant, M.H. (2002) Confocal laser scanning microscopy (CLSM) study of hepatocytes cultured on collagen films and gels. European Cells and Materials, 4 (2). pp. 72-73. ISSN 1473-2262

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Abstract

Summary of paper describing the process whereby primary hepatocyte cultures form an integral part of many hybrid artificial liver designs, and the extracellular matrix environment of the cultures is an important factor for optimal expression of hepatocyte-specific phenotype. This study investigates the effect of incorporating 20% chondroitin-6-sulphate (Ch6SO4), a glycosaminoglycan (GAG), into collagen films and gels, and crosslinking the films and the gels with 1,6-diaminohexane (DAH) on the viability of hepatocytes cultured for 48 hours.