Picture of a black hole

Strathclyde Open Access research that creates ripples...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of research papers by University of Strathclyde researchers, including by Strathclyde physicists involved in observing gravitational waves and black hole mergers as part of the Laser Interferometer Gravitational-Wave Observatory (LIGO) - but also other internationally significant research from the Department of Physics. Discover why Strathclyde's physics research is making ripples...

Strathprints also exposes world leading research from the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

Cryopreserved hepatocyte monolayers

Stevenson, D. and Morgan, C. and Goldie, E.I. and Connel, G. and Grant, M.H. (2002) Cryopreserved hepatocyte monolayers. Biochemical Society Transactions, 30. p. 94. ISSN 0300-5127

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Successful cryopreservation of hepatocytes would be useful to the pharmaceutical industry and for bioartificial liver support systems. Unfortunately, cryopreservation of hepatocytes in suspension results in low attachment efficiency in culture upon thawing. To circumvent this problem, we have frozen rat hepatocytes as monolayers on collagen substrates. Hepatocytes prepared from male Sprague Dawley rats by collagenase perfusion were cultured (3*106 cells/60mm Petri dish) on collagen films (30mg/cm2) in 2ml Chee's medium containing 5% v/v foetal calf serum (FCS). 24h cultures were frozen in Chee's medium with 10% Dimethyl Sulphoxide and between 0-90% FCS at -708C for 24h.