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Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coliK12 and O157

Qi, J. and Xu, J. and Hou, Y. and Yu, J. (2002) Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coliK12 and O157. Nucleic Acids Research, 30 (20). pp. 4432-41. ISSN 0305-1048

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Abstract

We have sequenced the genome of Shigella flexneri serotype 2a, the most prevalent species and serotype that causes bacillary dysentery or shigellosis in man. The whole genome is composed of a 4 607 203 bp chromosome and a 221 618 bp virulence plasmid, designated pCP301. While the plasmid shows minor divergence from that sequenced in serotype 5a, striking characteristics of the chromosome have been revealed. The S.flexneri chromosome has, astonishingly, 314 IS elements, more than 7-fold over those possessed by its close relatives, the non-pathogenic K12 strain and enterohemorrhagic O157:H7 strain of Escherichia coli. There are 13 translocations and inversions compared with the E.coli sequences, all involve a segment larger than 5 kb, and most are associated with deletions or acquired DNA sequences, of which several are likely to be bacteriophage-transmitted pathogenicity islands. Furthermore, S.flexneri, resembling another human-restricted enteric pathogen, Salmonella typhi, also has hundreds of pseudogenes compared with the E.coli strains. All of these could be subjected to investigations towards novel preventative and treatment strategies against shigellosis.

Item type: Article
ID code: 5465
Keywords: genome, Shigella flexneri, pathogenicity, bioscience, physiology, Pharmacy and materia medica, Physiology
Subjects: Medicine > Pharmacy and materia medica
Science > Physiology
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
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Depositing user: Strathprints Administrator
Date Deposited: 28 Feb 2008
Last modified: 12 Mar 2012 10:43
URI: http://strathprints.strath.ac.uk/id/eprint/5465

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