McQueenie, Ross and Stevenson, Ross and Benson, Robert and MacRitchie, Neil and McInnes, Iain and Maffia, Pasquale and Faulds, Karen and Graham, Duncan and Brewer, James and Garside, Paul (2012) Detection of inflammation in vivo by surface-enhanced Raman scattering provides higher sensitivity than conventional fluorescence imaging. Analytical Chemistry, 84 (14). pp. 5968-5975. ISSN 0003-2700Full text not available in this repository. (Request a copy from the Strathclyde author)
The detection of inflammatory changes is a key aim for the early diagnosis and treatment of several autoimmune, infectious, and metastatic diseases. While surface-enhanced Raman scattering (SERS) has the capability to provide noninvasive, in vivo imaging at sufficient depth to achieve this goal, this approach has not been exploited in the study of inflammation. SERS-active nanoparticles were coded with a unique Raman signal that was protected under a wide range of conditions and stimuli. To detect early-stage inflammation, gold nanoparticle clusters containing Raman-active molecules were conjugated to intercellular adhesion molecule 1- (ICAM-1-) specific monoclonal antibodies. SERS allowed noninvasive measurement of ICAM-1 expression in vivo with twice the sensitivity of two photon fluorescence. This is the first time SERS has been used for in vivo detection of inflammation and is a major advance in the ever-growing toolkit of approaches for use in noninvasive, next generation in vivo imaging.
|Keywords:||fluorescence imaging , Raman scattering , inflammation, Therapeutics. Pharmacology|
|Subjects:||Medicine > Therapeutics. Pharmacology|
|Department:||Faculty of Science > Physics|
Faculty of Science > Pure and Applied Chemistry
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
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|Depositing user:||Pure Administrator|
|Date Deposited:||27 Aug 2012 10:39|
|Last modified:||10 Dec 2013 10:05|
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