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Changes in protein expression associated with chronic in vitro exposure of hexavalent chromium to osteoblasts and monocytes: a proteomic approach

Raghunathan, Vijay Krishna and Grant, M.H. and Ellis, E. (2010) Changes in protein expression associated with chronic in vitro exposure of hexavalent chromium to osteoblasts and monocytes: a proteomic approach. Journal of Biomedical Materials Research Part A, 92A (2). pp. 615-625. ISSN 1549-3296

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Abstract

Cr (VI) is a well-recognized environmental toxin and carcinogen. It is known to be released from orthopedic metal implants in-situ by biocorrosion and is speculated to play a role in periprosthetic osteolysis. It is hence essential to understand its long-term biological effects. We have assessed the in vitro responses of osteoblasts and monocytes to chronic exposure (3 weeks) to Cr (VI), at concentrations that have been measured in patients with metal implants, using two-dimensional gel electrophoresis. Cr (VI) exposure resulted in a differential time-dependent regulation of glycolytic, stress, and cytoskeletal proteins. The proteins that have been found to be altered in expression play an essential role in normal cellular functioning such as energy metabolism, cell signaling, and proliferation. The results highlight the complex molecular changes that occur in both cell types with long-term exposure to Cr and may be useful in establishing a series of clinically useful biomarkers to monitor long-term use of metallic implants.

Item type: Article
ID code: 18408
Keywords: chronic in vitro toxicity, hexavalent chromium, monocytes, osteoblasts, metal orthopaedic implants, Bioengineering, Engineering (General). Civil engineering (General), Medicine(all)
Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Technology > Engineering (General). Civil engineering (General)
Department: Faculty of Engineering > Bioengineering
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
Depositing user: Ms Ashley Urie
Date Deposited: 26 Mar 2010 14:48
Last modified: 27 Mar 2014 08:51
URI: http://strathprints.strath.ac.uk/id/eprint/18408

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