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Single molecule level detection of allophycocyanin by surface enhanced resonance raman scattering

McGuinness, C.D and Macmillan, A.M. and Karolin, J. and Smith, W.E. and Graham, D. and Pickup, J.C. and Birch, D.J.S. (2007) Single molecule level detection of allophycocyanin by surface enhanced resonance raman scattering. Analyst, 132 (7). pp. 633-634. ISSN 0003-2654

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Abstract

Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm-1 band was 1 × 10-13 mol l-1, compared to 2 × 10-12 mol l-1 for the fluorescence peak at 660 nm.