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The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

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Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS)

Douglas, P. and Stokes, R.J. and Graham, D. and Smith, W.E. (2008) Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS). Analyst, 133 (6). pp. 791-796. ISSN 0003-2654

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Abstract

A micro-bead sandwich assay for P38 mitogen-activated protein kinase using surface enhanced resonance Raman spectroscopy (SERRS) detection is reported. Monoclonal capture antibodies were immobilised on a solid phase of magnetic micro-beads with secondary detection using a rhodamine-labelled antibody. Quantitative SERRS detection of the secondary antibody was possible with a limit of detection of 9.5 × 10−12 mol dm−3. The sandwich assay was quantitative and sensitive to 6 ng ml−1. The mechanism of the SERRS detection in the immunoassay was investigated. The addition of SERRS aggregating agents causes the dissociation of the immuno-complex from the magnetic beads. Scanning electron microscopy images indicate that the colloidal suspension rather than adsorbed silver nanoparticles on the beads provide the SERRS signals, that the aggregate size is partially controlled and that there is some inhomogeneity in the distribution of organic matter on the nanoscale. (Abstract from: http://www.swetswise.com/eAccess/viewAbstract.do?articleID=36962529)