Picture of a black hole

Strathclyde Open Access research that creates ripples...

The Strathprints institutional repository is a digital archive of University of Strathclyde's Open Access research outputs. Strathprints provides access to thousands of research papers by University of Strathclyde researchers, including by Strathclyde physicists involved in observing gravitational waves and black hole mergers as part of the Laser Interferometer Gravitational-Wave Observatory (LIGO) - but also other internationally significant research from the Department of Physics. Discover why Strathclyde's physics research is making ripples...

Strathprints also exposes world leading research from the Faculties of Science, Engineering, Humanities & Social Sciences, and from the Strathclyde Business School.

Discover more...

Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry

Wei, L.Q. and Watson, D.G. and Fearnley, J. (2008) Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry. Journal of Food Composition and Analysis, 21 (1). pp. 78-83. ISSN 0889-1575

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

The analysis of sugars in the natural products propolis and bee pollen was carried by high-performance thin layer chromatography (HPTLC), high-performance ligand exchange chromatography with pulsed amperometric detection (HPLEC-PAD) and HPLEC coupled to liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) in the positive ion mode. Eleven samples of propolis were analysed, most of which contained no sugars or only traces of sugars. Similar analysis was performed on five samples of bee pollen which were found to contain glucose, fructose and sucrose. These sugars were quantified by HPLEC-PAD. LC-ESI-MS analysis also indicated small amounts of a trisaccharide in the samples. Calibration curves were linear in the range 0.005-0.5 mg/ml and the LOD for all the sugars was <0.005 mg/ml. The positive ESI-MS of the sugars extracted from bee pollen yielded mainly Ca2+ adducts of sugars, which afforded good sensitivity for their detection.