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Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry

Wei, L.Q. and Watson, D.G. and Fearnley, J. (2008) Analysis of sugars in bee pollen and propolis by ligand exchange chromatography in combination with pulsed amperometric detection and mass spectrometry. Journal of Food Composition and Analysis, 21 (1). pp. 78-83. ISSN 0889-1575

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Abstract

The analysis of sugars in the natural products propolis and bee pollen was carried by high-performance thin layer chromatography (HPTLC), high-performance ligand exchange chromatography with pulsed amperometric detection (HPLEC-PAD) and HPLEC coupled to liquid chromatography-electrospray ionisation mass spectrometry (LC-ESI-MS) in the positive ion mode. Eleven samples of propolis were analysed, most of which contained no sugars or only traces of sugars. Similar analysis was performed on five samples of bee pollen which were found to contain glucose, fructose and sucrose. These sugars were quantified by HPLEC-PAD. LC-ESI-MS analysis also indicated small amounts of a trisaccharide in the samples. Calibration curves were linear in the range 0.005-0.5 mg/ml and the LOD for all the sugars was <0.005 mg/ml. The positive ESI-MS of the sugars extracted from bee pollen yielded mainly Ca2+ adducts of sugars, which afforded good sensitivity for their detection.

Item type: Article
ID code: 7601
Keywords: sugars, propolis, bee pollen, ligand exchange chromatography, pulsed amperometric detection, Pharmacy and materia medica, Food Science
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 25 Mar 2009 14:42
    Last modified: 04 Sep 2014 18:31
    URI: http://strathprints.strath.ac.uk/id/eprint/7601

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