Zhang, T. and Watson, D.G. and Tettey, J.N.A. and Stearns, A.T. and Binning, A.R. and Payne, C.J. (2007) Determination of vancomycin in serum by liquid chromatography-high resolution full scan mass spectrometry. Journal of Chromatography B, 857 (2). pp. 352-356. ISSN 1570-0232Full text not available in this repository. (Request a copy from the Strathclyde author)
A liquid chromatography-mass spectrometry (LC-MS) method was developed for the analysis of vancomycin (VCM) in human serum. The method was based on full scan data with extracted ions for the accurate masses of VCM and the atenolol internal standard obtained by Fourier transform MS. VCM was extracted from serum using strong cation exchange (SCX) solid phase extraction (SPE). The method was found to be linear in the range 0.05-10 μg/ml, which was adequate for quantification of VCM in serum samples, with a limit of quantification (LOQ) of 0.005 μg/ml and a limit of detection (LOD) of 0.001 μg/ml. Intra-day precision (n = 5) was ±3.5%, ±2.5%, ±0.7% at 0.05, 0.5 and 5 μg/ml, respectively. Inter-day precision (n = 5) was ±7.6%, ±6.4%, ±3.9% at 0.05, 0.5 and 5 μg/ml, respectively. The process efficiency for VCM was in the range 89.2-98.1% with the recovery for the atenolol internal standard (IS) being 97.3%. The method was used to determine VCM levels in patients during peri-operative infusion of the drug, which was found to result in drug levels within the required therapeutic window.
|Keywords:||vancomycin, serum, fourier transform mass spectrometry, ion suppression, pharmacology, Pharmacy and materia medica, Biochemistry, Cell Biology, Analytical Chemistry, Clinical Biochemistry|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||24 Mar 2009 16:56|
|Last modified:||06 Jan 2017 06:07|