Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Oral delivery of tetanus toxoid using vesicles containing bile salts (bilosomes) induces significant systemic and mucosal immunity

Alexander, J. and Mann, J.F.S. and Scales, H.E. and Shakir, E. and Carter, K.C. and Mullen, A. and Ferro, V.A. (2006) Oral delivery of tetanus toxoid using vesicles containing bile salts (bilosomes) induces significant systemic and mucosal immunity. Methods, 38 (2). pp. 90-95. ISSN 1046-2023

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Protein antigens administered via the oral route are exposed to a hostile environment in the gastrointestinal tract, consisting of digestive enzymes and a range of pH (1-7.5). Using a delivery system can afford protection to entrapped components against degradation and permit delivery of antigen to the cells responsible for generating local and systemic immune responses. In this comparative study, mice were immunised orally with tetanus toxoid (40 or 200 μg dose/mouse, four doses in total) entrapped in non-ionic surfactant vesicles formulated with bile salts (bilosomes). The higher entrapped dose (BV-TT, 200 μg) induced IgG1 by study week 3 to similar levels to those observed with subcutaneous un-entrapped TT at the lower (<50 μg) dose. However, both bilosome formulations (BV-TT, low, and high doses), though not un-entrapped TT, caused a rise in the numbers of IgA positive plasma cells observed in the small intestine, primarily in the first 15 cm of the small intestine.

Item type: Article
ID code: 7575
Keywords: oral delivery, vaccine, tetanus toxoid, antibody, immunohistochemistry, Pharmacy and materia medica
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences > Immunology
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 12 Apr 2009 16:29
    Last modified: 12 Mar 2012 10:47
    URI: http://strathprints.strath.ac.uk/id/eprint/7575

    Actions (login required)

    View Item