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Damage to F-actin and cell death induced by chromium VI and nickel in primary monolayer cultures of rat hepatocytes

Gunaratnam, M. and Grant, M.H. (2004) Damage to F-actin and cell death induced by chromium VI and nickel in primary monolayer cultures of rat hepatocytes. Toxicology in Vitro, 18 (3). pp. 245-253. ISSN 0887-2333

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Abstract

The toxicity of hexavalent chromium and nickel was investigated using primary cultures of hepatocytes as an in vitro system. Cr VI and Ni are widely used in the steel and orthopaedic implant industry. Although their toxicity has been extensively investigated, the mechanism(s) of action is/are not fully understood. Monolayer cultures of hepatocytes (105 cells/cm2) were exposed to various concentrations of Cr VI and Ni for 24 h. Cells were stained with phalloidin-FITC for the detection of the cytoskeletal component, F-actin, and Annexin V-FITC and propidium iodide for the detection of the mode of cell death. Exposure of cells to Cr VI (1, 5, 10 and 50 μImage ) resulted in the loss of the cell cytoskeleton, and this was accompanied by membrane blebbing and shrinking of the cell. Ni, on the other hand, induced detectable damage to the cytoskeleton only at 500 μImage . Staining of the cells with Annexin V and propidium iodide showed that Cr VI induces apoptosis at low concentrations (5 μImage ), and necrosis at higher concentrations (25 and 50 μImage ). Ni almost exclusively induced necrosis at 500 μImage with very few cells undergoing apoptosis. Below this concentration it had no discernable effect on hepatocytes. Damage to the cell cytoskeleton caused by Cr VI may be an early indication of apoptosis in hepatocytes.

Item type: Article
ID code: 6770
Keywords: chromium, nickel, apoptosis, cytoskeleton, hepatocytes, bioengineering, Bioengineering, Human anatomy, Toxicology
Subjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Science > Human anatomy
Department: Faculty of Engineering > Bioengineering
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 28 Aug 2008
    Last modified: 04 Sep 2014 15:53
    URI: http://strathprints.strath.ac.uk/id/eprint/6770

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