Strathprints logo
Strathprints Home | Open Access | Browse | Search | User area | Copyright | Help | Library Home | SUPrimo

Comparative phenotype and immunogenicity of freshly isolated and immortalized rat hepatocytes

Kaulek, V. and Sass, P. and Alexandre, E. and Grant, M.H. and Richert, L. and Jaeck, D. and Tiberghien, P. and Wolf, P. and Azimzadeh, A. (2001) Comparative phenotype and immunogenicity of freshly isolated and immortalized rat hepatocytes. Cell Transplantation, 10 (8). pp. 739-747. ISSN 0963-6897

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

Immortalized hepatocytes are an attractive cell source for hepatocyte transplantation and gene transfer. We compared the phenotype and immunogenicity of freshly isolated (FIH) and immortalized (IMH) rat hepatocytes. Effect of culture and proinflammatory cytokines (TNF-agr, IFN-ggr) was studied on phenotype. FIH were isolated by collagenase digestion. Two SV40 immortalized hepatocyte cell lines were tested (RH1 and P9). Immunophenotyping was performed by FACS analysis using anti-rat-specific antibodies. Immunogenicity was evaluated by a mixed lymphocyte hepatocyte reaction (MLHR). FIH suspension was an almost homogeneous parenchymal cell population with few (1-2%) CD8+ cells. FIH showed a positive staining for ICAM-1 (20-35%) and for Class I (RT1A, 30-60%) but no staining for Class II (RT1B). After 48 h of culture, the already ICAM-1-positive cells were more strongly stained and additionally 3.6% of the cells (possibly endothelial cells) were Class II positive. IMH showed a consistent expression of Class I (93-97%) and ICAM-1 (95-97%) but no expression of Class II. Culture of IMH for 48 h had no effect on Class II expression but increased ICAM-1 expression. Addition of TNF-agr at 1000 UI/ml to cultures of FIH or IMH increased Class I and ICAM-1 expression whereas IFN-ggr (50 or 1000 UI/ml) had no evident effect. Hepatocyte immunogenicity, assessed in MLHR and appreciated by the stimulation index (SI) test/SI syngeneic control, was similar for IMH (RH1: 2.68 ± 0.89; P9: 2.37 ± 0.78) and FIH (2.52 ± 0.18). In conclusion, despite some quantitative immunophenotypic differences, FIH and IMH induced the same proliferation rate of allogeneic T lymphocytes. Thus, immortalized hepatocytes may constitute an appropriate cellular model to study the prevention of hepatocyte rejection by gene transfer.

Item type: Article
ID code: 3696
Keywords: transplantation, cells, bioengineering, biomechanics, hepatocytes, gene transfer, Technology (General), Other systems of medicine, Physiology, Biomedical Engineering, Cell Biology, Transplantation
Subjects: Technology > Technology (General)
Medicine > Other systems of medicine
Science > Physiology
Department: Faculty of Engineering > Bioengineering
Related URLs:
Depositing user: Strathprints Administrator
Date Deposited: 05 Jul 2007
Last modified: 04 Sep 2014 10:23
URI: http://strathprints.strath.ac.uk/id/eprint/3696

Actions (login required)

View Item