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An RNA Aptamer that Induces Transcription

Hunsicker, A. and Steber, M. and Mayer, G. and Meitert, J. and Klotzsche, M. and Blind, M. and Hillen, W. and Berens, C. (2009) An RNA Aptamer that Induces Transcription. Chemistry and Biology, 16. pp. 173-180. ISSN 1074-5521

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Abstract

We identified an RNA aptamer that induces TetR-controlled gene expression in Escherichia coli when expressed in the cell. The aptamer was found by a combined approach of in vitro selection for TetR binding and in vivo screening for TetR induction. The smallest active aptamer folds into a stem-loop with an internal loop interrupting the stem. Mutational analysis in vivo and in-line probing in vitro reveal this loop to be the protein binding site. The TetR-inducing activity of the aptamer directly correlates with its stability and the best construct is as efficient as the natural inducer tetracycline. Because of its small size, high induction efficiency, and the stability of the TetR aptamer under in vivo conditions, it is well suited to be an alternative RNA-based inducer of TetR-controlled gene expression.

Item type: Article
ID code: 26094
Notes: Strathprints' policy is to record up to 8 authors per publication, plus any additional authors based at the University of Strathclyde. More authors may be listed on the official publication than appear in the Strathprints' record.
Keywords: TN10 tet repressor, in-vitro, selection, binding, recognition, operator, ligands, system, motif, vivo, Genetics, Biochemistry, Drug Discovery, Molecular Medicine, Molecular Biology, Pharmacology, Clinical Biochemistry
Subjects: Science > Natural history > Genetics
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 23 Aug 2010 12:44
    Last modified: 05 Sep 2014 05:16
    URI: http://strathprints.strath.ac.uk/id/eprint/26094

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