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Variability of invitro migration of human-leukocytes

Pattison, J.D. and Filer, D. and Anderson, W.G. and Gettinby, G. and Anderson, J.M. (1981) Variability of invitro migration of human-leukocytes. Journal of Clinical and Laboratory Immunology, 5 (3). pp. 191-194. ISSN 0141-2760

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Abstract

A comparison of the capillary method (CM) and the agarose microdroplet method (AMM), using the same materials, for measurement of human leucocyte migration in vitro yielded similar large degrees of variability. Between fifteen and twenty-six replicate cultures were established for each method in fourteen subjects The range of the coefficients of variation were .7-28.8% (CM) and 7.2-19.3% AMM). Most of 115 publications reviewed describe only three or four replicates. Their results relating to host responses in the fields of cancer, tissue transplantation and several chronic diseases should be interpreted with regard to the variability of the system. The use of human leucocyte migration in vitro for any purpose should incorporate more stringent statistical control of sample size than has been customary. The use of two standard statistical formulae is proposed as this control.

Item type: Article
ID code: 17844
Keywords: capillary method, agarose microdroplet method, comparison, materials, measurement, human leucocyte migration, in vitro, variability, Probabilities. Mathematical statistics, Immunology
Subjects: Science > Mathematics > Probabilities. Mathematical statistics
Department: Faculty of Science > Mathematics and Statistics
Faculty of Engineering > Design, Manufacture and Engineering Management
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    Depositing user: Strathprints Administrator
    Date Deposited: 14 May 2010 11:29
    Last modified: 05 Sep 2014 00:25
    URI: http://strathprints.strath.ac.uk/id/eprint/17844

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