Rolinski, O.J. and Birch, D.J.S. (2000) Determination of acceptor distribution from fluorescence resonance energy transfer: theory and simulation. Journal of Chemical Physics, 112 (20). pp. 8923-8933. ISSN 0021-9606
Full text not available in this repository. (Request a copy from the Strathclyde author)Abstract
A new method for determining the donor-acceptor distribution function in fluorescence resonance energy transfer (FRET) systems is presented. The approach is based on time-resolved fluorescence experiments with nanosecond resolution. Potential applications of this method include: determination of the morphology of porous materials (e.g., polymers, sol-gels, resins, etc.), monitoring processes occurring on the nanometer scale including biomolecules labeled with the donor/acceptor species, and FRET sensors based on competitive binding. In this paper a theoretical derivation of the method is presented and the method is tested in a series of numerical simulations. The experimental conditions regarding this approach are discussed and its applicability to real measurement systems is demonstrated
| Item type: | Article |
|---|---|
| ID code: | 17495 |
| Keywords: | FRET, fluorescence resonance energy transfer, Physics |
| Subjects: | Science > Physics |
| Department: | Faculty of Science > Physics |
| Related URLs: | |
| Depositing user: | Strathprints Administrator |
| Date Deposited: | 05 May 2010 16:39 |
| Last modified: | 12 Mar 2012 11:07 |
| URI: | http://strathprints.strath.ac.uk/id/eprint/17495 |
Actions (login required)
| View Item |