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Determination of acceptor distribution from fluorescence resonance energy transfer: theory and simulation

Rolinski, O.J. and Birch, D.J.S. (2000) Determination of acceptor distribution from fluorescence resonance energy transfer: theory and simulation. Journal of Chemical Physics, 112 (20). pp. 8923-8933. ISSN 0021-9606

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Abstract

A new method for determining the donor-acceptor distribution function in fluorescence resonance energy transfer (FRET) systems is presented. The approach is based on time-resolved fluorescence experiments with nanosecond resolution. Potential applications of this method include: determination of the morphology of porous materials (e.g., polymers, sol-gels, resins, etc.), monitoring processes occurring on the nanometer scale including biomolecules labeled with the donor/acceptor species, and FRET sensors based on competitive binding. In this paper a theoretical derivation of the method is presented and the method is tested in a series of numerical simulations. The experimental conditions regarding this approach are discussed and its applicability to real measurement systems is demonstrated

Item type: Article
ID code: 17495
Keywords: FRET, fluorescence resonance energy transfer, Physics, Physics and Astronomy(all), Physical and Theoretical Chemistry
Subjects: Science > Physics
Department: Faculty of Science > Physics
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 05 May 2010 16:39
    Last modified: 05 Sep 2014 01:11
    URI: http://strathprints.strath.ac.uk/id/eprint/17495

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