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Effect of protease-activated receptor-2 deficiency on allergic dermatitis in the mouse ear

Takizawa, T. and Wada, Y. and Matsumoto, J. and Tamiya, M. and Smith, A.J.H. and Plevin, R.J. and Kanke, T. and Saino, Y. (2002) Effect of protease-activated receptor-2 deficiency on allergic dermatitis in the mouse ear. Japanese Journal of Pharmacology, 88 (1). pp. 77-84. ISSN 0021-5198

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Abstract

To investigate the involvement of protease-activated receptor-2 (PAR-2) in allergic dermatitis, we generated PAR-2-deficient (PAR-2−/−) mice. Ear thickness, contact hypersensitivity (CH) induced by topical application of picryl chloride (PC) or oxazolone (Ox) after sensitization, and vascular permeability after ear passive cutaneous anaphylaxis (PCA) were compared between wild-type (WT) and PAR-2−/− mice. Ear thickness was almost the same in untreated WT and PAR-2−/− mice. Topical application of PC or Ox thickened the ears at 6, 24 and 48 h after challenge with a peak at 24 h in WT mice. In PAR-2−/− mice, the ear swelling induced by both PC and Ox was suppressed at every time point, and significant inhibition was found at 24 h in PC-induced CH and at 24 and 48 h in Ox-induced CH. Histopathological observation of the ears at 24 h after challenge revealed that PC- or Ox-induced ear edema and infiltration of inflammatory cells in WT mice were greatly attenuated in PAR-2−/− mice. The vascular permeability in the ears after PCA was not different between WT and PAR-2−/− mice. These results strongly suggest that PAR-2 plays a crucial role in type IV allergic dermatitis but not in type I allergic dermatitis.

Item type: Article
ID code: 17411
Notes: Strathprints' policy is to record up to 8 authors per publication, plus any additional authors based at the University of Strathclyde. More authors may be listed on the official publication than appear in the Strathprints' record.
Keywords: protease-activated receptor-2, knockout mice, Allergic dermatitis, contact hypersensitivity, passive cutaneous anaphylaxis, Pharmacy and materia medica, Pharmacology
Subjects: Medicine > Pharmacy and materia medica
Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 15 Apr 2010 11:50
    Last modified: 05 Sep 2014 01:16
    URI: http://strathprints.strath.ac.uk/id/eprint/17411

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