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SERRS-based enzymatic probes for the detection of protease activity

Ingram, A. and Byers, L. and Faulds, K. and Moore, B.D. and Graham, D. (2008) SERRS-based enzymatic probes for the detection of protease activity. Journal of the American Chemical Society, 130 (36). pp. 11846-11847. ISSN 0002-7863

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Abstract

Measurement of protease activity, for the first time using SERRS as a detection method, is reported herein. Synthetic introduction of phenylalanine to a benzotriazole azo dye allows the SERRS response to be 'switched off' and subsequent exposure to protease restores the SERRS response. The substrates exhibit varying reactivity for a range of proteases and allow for in situ, real-time analysis of protease reactivity. A limit of detection for one protease, Subtilisin carlsberg, was investigated and was established to be 50 ng ml−1.

Item type: Article
ID code: 17043
Keywords: SERRS-based, enzymatic probes, detection, protease activity, Science (General), Therapeutics. Pharmacology, Pharmacy and materia medica, Biochemistry, Colloid and Surface Chemistry, Chemistry(all), Catalysis
Subjects: Science > Science (General)
Medicine > Therapeutics. Pharmacology
Medicine > Pharmacy and materia medica
Department: Faculty of Science > Pure and Applied Chemistry
Related URLs:
    Depositing user: Strathprints Administrator
    Date Deposited: 20 Apr 2010 10:41
    Last modified: 05 Sep 2014 02:38
    URI: http://strathprints.strath.ac.uk/id/eprint/17043

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