Picture of virus under microscope

Research under the microscope...

The Strathprints institutional repository is a digital archive of University of Strathclyde research outputs.

Strathprints serves world leading Open Access research by the University of Strathclyde, including research by the Strathclyde Institute of Pharmacy and Biomedical Sciences (SIPBS), where research centres such as the Industrial Biotechnology Innovation Centre (IBioIC), the Cancer Research UK Formulation Unit, SeaBioTech and the Centre for Biophotonics are based.

Explore SIPBS research

Determination of thiol concentrations in hemolysate by resonance raman spectrometry

Banford, J.C. and Brown, D.H. and McConnell, A.A. and McNeill, C.J. and Smith, W.E. and Hazelton, R.A. and Sturrock, R.D. (1982) Determination of thiol concentrations in hemolysate by resonance raman spectrometry. Analyst, 107 (1271). pp. 195-199. ISSN 0003-2654

Full text not available in this repository. (Request a copy from the Strathclyde author)

Abstract

A simple and sensitive method for determining the thiol concentration in haemolysate, using the resonance Raman spectrum of the product of the reaction of the lysate with 5,5-dithiobis(2-nitrobenzoic acid)(Ellman's reagent), is described. The method uses a signal due to the haem ring of haemoglobin as an internal calibrant. In six separate determinations of the thiol concentrations in the lysate from a normal male volunteer, the average thiol concentration was 1 820 µM and the relative standard deviation was 4.5%. Standard addition of glutathione to four lysate samples confirmed this result and indicated an acceptable precision. To obtain a comparison with another technique, clear lysates were prepared by precipitation of haemoglobin and addition of sufficient glutathione to maintain thiol levels comparable to those of coloured lysates. Agreement between the resonance Raman method and a spectrophotometric method was acceptable in this instance. The method is relatively free from the standard interferences found for spectrophotometric assays and is selective. The mean value for the lysate from six normal male volunteers (1920 ± 88 µM) and from seven patients with rheumatoid arthritis (3240 ± 774 µM) were significantly different, suggesting that this measurement may have relevance in the study of disease processes.