Bengs, F. and Scholz, A. and Kuhn, D. and Wiese, M. (2005) LmxMPK9, a mitogen-activated protein kinase homologue affects flagellar length in leishmania mexicana. Molecular Microbiology, 55 (5). pp. 1606-1615. ISSN 0950-382XFull text not available in this repository. (Request a copy from the Strathclyde author)
Components of mitogen-activated signal transduction pathways have been shown to be involved in flagellum biogenesis and maintenance. A mitogen-activated protein kinase homologue, designated LmxMPK9 from Leishmania mexicana, has been recently identified in a homology screen and its mRNA found to be present in all life stages. Three different splice-addition sites were used for mRNA maturation in trans-splicing in the different life stages. However, here we show that LmxMPK9 protein is exclusively found in the promastigote stage. Recombinant expression of LmxMPK9 in Escherichia coli and kinase assays revealed a temperature optimum at 27 degrees C, the optimal growth temperature for L. mexicana promastigotes, and a preference for manganese to promote substrate phosphorylation of myelin basic protein. A deletion mutant for the single-copy gene revealed significantly elongated flagella, whereas overexpression led to a subpopulation with rather short to no flagella suggesting a role for LmxMPK9 in flagellar morphogenesis.
|Keywords:||Leishmania mexicana, mitogen-activated protein kinase , flagellar length , Pharmacy and materia medica, Molecular Biology, Microbiology|
|Subjects:||Medicine > Pharmacy and materia medica|
|Department:||Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences|
|Depositing user:||Strathprints Administrator|
|Date Deposited:||23 Jun 2011 12:06|
|Last modified:||26 Aug 2016 02:12|