Genome mining of amylases and amylase inhibitors from Streptomyces

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Lawaetz, Andreas C. and Gannon, Alexa and Hoskisson, Paul A. (2026) Genome mining of amylases and amylase inhibitors from Streptomyces. Microbial Genomics, 12 (6). 001747. ISSN 2057-5858 (https://doi.org/10.1099/mgen.0.001747)

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Abstract

Most antibiotics are derived from Streptomyces bacteria and produced industrially via fermentations relying on food-grade feedstocks, which has a substantial environmental impact. Efficient utilisation of starch-rich organic wastes such as bread and potato waste as alternative feedstocks remains limited by incomplete knowledge of starch metabolism in Streptomyces. Here, a genus-wide analysis of 295 Streptomyces strains was performed, identifying 645 amylases grouped into pullulanases, α-1,4-amylases, and cyclomaltodextrin-like amylases, alongside biosynthetic gene clusters encoding amylase inhibitors such as acarbose and tendamistat. Structural and sequence analyses revealed that inhibitor resistance arises from subtle modifications in the α-amylase catalytic pocket. This comprehensive analysis of amylases and inhibitors provides a foundation for engineering inhibitor-resistant enzymes tailored to diverse starch substrates, facilitating the development of sustainable, starch-based Streptomyces fermentations.

ORCID iDs

Lawaetz, Andreas C. ORCID logoORCID: https://orcid.org/0000-0001-5035-8420, Gannon, Alexa and Hoskisson, Paul A. ORCID logoORCID: https://orcid.org/0000-0003-4332-1640;
CORE (COnnecting REpositories)