Chronically implantable μ LED arrays for optogenetic cortical surface stimulation in mice

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Greer, Ryan and Verdier, Antonin and Butt, Emma and Cheng, Yunzhou and Callas, Ella and McAlinden, Niall and Aniorte, Alicia and Mabrouk Kakaouia, Eya and Pereyra, Magdalena and Dawson, Martin D. and Bathellier, Brice and Mathieson, Keith (2026) Chronically implantable μ LED arrays for optogenetic cortical surface stimulation in mice. Nature Communications, 17 (1). 878. ISSN 2041-1723 (https://doi.org/10.1038/s41467-025-68191-5)

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Abstract

Cortical implants are a proven clinical neurotechnology with the potential to transform our understanding of cognitive processes. These processes rely on complex neuronal networks that are difficult to selectively probe or stimulate. Optogenetics offers cell-type specificity, but achieving the density and coverage required for chronic, high-resolution modulation remains a challenge. We fabricated 100-element μLED arrays (200 μm pixel pitch, 2 × 2 mm2 footprint) coupled into chronically implantable systems for optogenetic stimulation of the mouse cortex. The μLEDs remain stable for over 300 hours continuous operation time in vivo, allowing for months-long chronic experiments. Simultaneous electrophysiology recordings confirmed robust neuronal responses at low μLED drive currents (< 5 mA), minimising thermal effects. Here we show that our device can be chronically implanted in freely-behaving mice and drive behavioural outcomes from spatiotemporal, patterned, optogenetic stimulation of auditory cortical circuits.

ORCID iDs

Greer, Ryan, Verdier, Antonin, Butt, Emma ORCID logoORCID: https://orcid.org/0000-0003-1622-3818, Cheng, Yunzhou ORCID logoORCID: https://orcid.org/0000-0001-7366-4893, Callas, Ella, McAlinden, Niall ORCID logoORCID: https://orcid.org/0000-0002-0369-8788, Aniorte, Alicia, Mabrouk Kakaouia, Eya, Pereyra, Magdalena, Dawson, Martin D. ORCID logoORCID: https://orcid.org/0000-0002-6639-2989, Bathellier, Brice and Mathieson, Keith ORCID logoORCID: https://orcid.org/0000-0002-9517-8076;
CORE (COnnecting REpositories)