Development and testing of a LED array microscope for multimodal diffraction-limited and super-resolution biological imaging

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Walker, Lewis and Copeland, Laura and Shaw, Michael and McConnell, Gail (2025) Development and testing of a LED array microscope for multimodal diffraction-limited and super-resolution biological imaging. In: Microscience Microscopy Congress 2025 incorporating EMAG 2025, 2025-06-30 - 2025-07-03.

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Abstract

The integration of a low-cost LED array into a standard microscope enables multimodal visualisation of biological samples under brightfield, darkfield, phase, and fluorescence contrast, Rheinberg illumination, and Fourier Ptychographic Microscopy (FPM). FPM reconstructs widefield, high resolution images by computationally combining multiple images captured under oblique illumination, therefore overcoming traditional resolution limitations. More than a two-fold improvement in resolution was achieved over a 2.2 mm^2 field of view (FOV) using a standard inverted microscope with an integrated light-emitting diode (LED) array. Multimodal imaging, including FPM, was performed on live cell specimens using a PL FLUOTAR 10x/0.3 PH1 objective lens.

ORCID iDs

Walker, Lewis ORCID logoORCID: https://orcid.org/0009-0008-0375-7793, Copeland, Laura ORCID logoORCID: https://orcid.org/0009-0008-7117-3626, Shaw, Michael and McConnell, Gail ORCID logoORCID: https://orcid.org/0000-0002-7213-0686;
CORE (COnnecting REpositories)