Electrochemiluminescent detection of donepezil

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Ameen, Anaam and Cavanagh, Jay and Shum, P.H. and Blake, Rowan and Brown, Kelly and Allan, Pamela and Dennany, Lynn (2026) Electrochemiluminescent detection of donepezil. Journal of Electroanalytical Chemistry, 1001. 119714. ISSN 0022-0728 (https://doi.org/10.1016/j.jelechem.2025.119714)

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Abstract

The move towards personalised precision medicine, requires monitoring of drug therapies as well as progression makers. Indeed, the monitoring of drug therapies is essential for the continuing evolution of precision medicine in conjunction with drug development and targeted treatment strategies. This study presents the feasibility of electrochemiluminescence (ECL) for the detection of donepezil hydrochloride (DZ) as a proof-of-concept for point-of-care applications within human pooled serum and artificial urine. ECL has the potential to be an easily understood point-of-care sensor increasing its potential for translation as the intensity of the emitted light is proportional to the target analyte concentration. This proof-of-concept demonstrated its ability to detect DZ over the clinically relevant concentration range of 0.5 to 1000 µM, and was effective at detecting 0.5, 1, 1.5 and 7.5 µM spiked into human pooled serum and artificial urine. While more development is still needed, this highlights the applicability of electrochemical sensors, including ECL based sensors, for point-of-care devices with the reduction of sample preparations improving its feasibility for clinical and point-of-care applications.

ORCID iDs

Ameen, Anaam, Cavanagh, Jay, Shum, P.H., Blake, Rowan, Brown, Kelly ORCID logoORCID: https://orcid.org/0000-0002-4608-7741, Allan, Pamela ORCID logoORCID: https://orcid.org/0000-0003-4989-2698 and Dennany, Lynn ORCID logoORCID: https://orcid.org/0000-0002-5481-1066;
CORE (COnnecting REpositories)