Translation microscopy (TRAM) for super-resolution imaging

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Qiu, Zhen and Wilson, Rhodri S and Liu, Yuewei and R Dun, Alison and Saleeb, Rebecca S and Liu, Dongsheng and Rickman, Colin and Frame, Margaret and Duncan, Rory R and Lu, Weiping (2016) Translation microscopy (TRAM) for super-resolution imaging. Scientific Reports, 6. 19993. ISSN 2045-2322 (https://doi.org/10.1038/srep19993)

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Abstract

Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel ground-truth DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology.

ORCID iDs

Qiu, Zhen ORCID logoORCID: https://orcid.org/0000-0002-0226-7855, Wilson, Rhodri S, Liu, Yuewei, R Dun, Alison, Saleeb, Rebecca S, Liu, Dongsheng, Rickman, Colin, Frame, Margaret, Duncan, Rory R and Lu, Weiping;
CORE (COnnecting REpositories)