SLING : a tool to search for linked genes in bacterial datasets
Horesh, Gal and Harms, Alexander and Fino, Cinzia and Parts, Leopold and Gerdes, Kenn and Heinz, Eva and Thomson, Nicholas Robert (2018) SLING : a tool to search for linked genes in bacterial datasets. Nucleic Acids Research, 46 (21). e128. ISSN 1362-4962 (https://doi.org/10.1093/nar/gky738)
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Abstract
Gene arrays and operons that encode functionally linked proteins form the most basic unit of transcriptional regulation in bacteria. Rules that govern the order and orientation of genes in these systems have been defined; however, these were based on a small set of genomes that may not be representative. The growing availability of large genomic datasets presents an opportunity to test these rules, to define the full range and diversity of these systems, and to understand their evolution. Here we present SLING, a tool to Search for LINked Genes by searching for a single functionally essential gene, along with its neighbours in a rule-defined proximity (https://github.com/ghoresh11/sling/wiki). Examining this subset of genes enables us to understand the basic diversity of these genetic systems in large datasets. We demonstrate the utility of SLING on a clinical collection of enteropathogenic Escherichia coli for two relevant operons: toxin antitoxin (TA) systems and RND efflux pumps. By examining the diversity of these systems, we gain insight on distinct classes of operons which present variable levels of prevalence and ability to be lost or gained. The importance of this analysis is not limited to TA systems and RND pumps, and can be expanded to understand the diversity of many other relevant gene arrays.
ORCID iDs
Horesh, Gal, Harms, Alexander, Fino, Cinzia, Parts, Leopold, Gerdes, Kenn, Heinz, Eva ORCID: https://orcid.org/0000-0003-4413-3756 and Thomson, Nicholas Robert;-
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Item type: Article ID code: 90658 Dates: DateEvent30 November 2018Published16 August 2018Published Online8 August 2018AcceptedSubjects: Science > Natural history > Genetics Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 23 Sep 2024 14:55 Last modified: 23 Sep 2024 14:55 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/90658