Colonization dynamics of extended-spectrum beta-lactamase-producing Enterobacterales in the gut of Malawian adults
Lewis, Joseph M. and Mphasa, Madalitso and Banda, Rachel and Beale, Mathew A. and Heinz, Eva and Mallewa, Jane and Jewell, Christopher and Faragher, Brian and Thomson, Nicholas R. and Feasey, Nicholas A. (2022) Colonization dynamics of extended-spectrum beta-lactamase-producing Enterobacterales in the gut of Malawian adults. Nature microbiology, 7 (10). pp. 1593-1604. ISSN 2058-5276 (https://doi.org/10.1038/s41564-022-01216-7)
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Abstract
Drug-resistant bacteria of the order Enterobacterales which produce extended-spectrum beta-lactamase enzymes (ESBL-Enterobacterales, ESBL-E) are global priority pathogens. Antimicrobial stewardship interventions proposed to curb their spread include shorter courses of antimicrobials to reduce selection pressure but individual-level acquisition and selection dynamics are poorly understood. We sampled stool of 425 adults (aged 16–76 years) in Blantyre, Malawi, over 6 months and used multistate modelling and whole-genome sequencing to understand colonization dynamics of ESBL-E. Models suggest a prolonged effect of antimicrobials such that truncating an antimicrobial course at 2 days has a limited effect in reducing colonization. Genomic analysis shows largely indistinguishable diversity of healthcare-associated and community-acquired isolates, hence some apparent acquisition of ESBL-E during hospitalization may instead represent selection from a patient’s microbiota by antimicrobial exposure. Our approach could help guide stewardship protocols; interventions that aim to review and truncate courses of unneeded antimicrobials may be of limited use in preventing ESBL-E colonization.
ORCID iDs
Lewis, Joseph M., Mphasa, Madalitso, Banda, Rachel, Beale, Mathew A., Heinz, Eva ORCID: https://orcid.org/0000-0003-4413-3756, Mallewa, Jane, Jewell, Christopher, Faragher, Brian, Thomson, Nicholas R. and Feasey, Nicholas A.;-
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Item type: Article ID code: 90647 Dates: DateEventOctober 2022Published5 September 2022Published Online25 July 2022AcceptedSubjects: Science > Microbiology Department: UNSPECIFIED Depositing user: Pure Administrator Date deposited: 23 Sep 2024 12:42 Last modified: 23 Sep 2024 12:42 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/90647