Assembly of the type II secretion system such as found in vibrio cholerae depends on the novel pilotin AspS
Dunstan, Rhys A. and Heinz, Eva and Wijeyewickrema, Lakshmi C. and Pike, Robert N. and Purcell, Anthony W. and Evans, Timothy J. and Praszkier, Judyta and Robins-Browne, Roy M. and Strugnell, Richard A. and Korotkov, Konstantin V. and Lithgow, Trevor (2013) Assembly of the type II secretion system such as found in vibrio cholerae depends on the novel pilotin AspS. PLOS Pathogens, 9 (1). e1003117. ISSN 1553-7366 (https://doi.org/10.1371/journal.ppat.1003117)
Preview |
Text.
Filename: Dunstan-etal-PLOSP-2013-Assembly-of-the-Type-II-Secretion-System.pdf
Final Published Version License: Download (2MB)| Preview |
Abstract
The Type II Secretion System (T2SS) is a molecular machine that drives the secretion of fully-folded protein substrates across the bacterial outer membrane. A key element in the machinery is the secretin: an integral, multimeric outer membrane protein that forms the secretion pore. We show that three distinct forms of T2SSs can be distinguished based on the sequence characteristics of their secretin pores. Detailed comparative analysis of two of these, the Klebsiella-type and Vibrio-type, showed them to be further distinguished by the pilotin that mediates their transport and assembly into the outer membrane. We have determined the crystal structure of the novel pilotin AspS from Vibrio cholerae, demonstrating convergent evolution wherein AspS is functionally equivalent and yet structurally unrelated to the pilotins found in Klebsiella and other bacteria. AspS binds to a specific targeting sequence in the Vibrio-type secretins, enhances the kinetics of secretin assembly, and homologs of AspS are found in all species of Vibrio as well those few strains of Escherichia and Shigella that have acquired a Vibrio-type T2SS.
ORCID iDs
Dunstan, Rhys A., Heinz, Eva ORCID: https://orcid.org/0000-0003-4413-3756, Wijeyewickrema, Lakshmi C., Pike, Robert N., Purcell, Anthony W., Evans, Timothy J., Praszkier, Judyta, Robins-Browne, Roy M., Strugnell, Richard A., Korotkov, Konstantin V. and Lithgow, Trevor;-
-
Item type: Article ID code: 90642 Dates: DateEvent10 January 2013Published20 November 2012AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 23 Sep 2024 12:01 Last modified: 23 Sep 2024 12:01 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/90642