Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes
Hegde, Shivanand and Brettell, Laura E. and Quek, Shannon and Etebari, Kayvan and Saldaña, Miguel A. and Asgari, Sassan and Coon, Kerri L. and Heinz, Eva and Hughes, Grant L. (2024) Aedes aegypti gut transcriptomes respond differently to microbiome transplants from field-caught or laboratory-reared mosquitoes. Environmental Microbiology, 26 (2). e16576. ISSN 1462-2912 (https://doi.org/10.1111/1462-2920.16576)
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Abstract
The mosquito microbiome is critical for host development and plays a major role in many aspects of mosquito biology. While the microbiome is commonly dominated by a small number of genera, there is considerable variation in composition among mosquito species, life stages, and geography. How the host controls and is affected by this variation is unclear. Using microbiome transplant experiments, we asked whether there were differences in transcriptional responses when mosquitoes of different species were used as microbiome donors. We used microbiomes from four different donor species spanning the phylogenetic breadth of the Culicidae, collected either from the laboratory or the field. We found that when recipients received a microbiome from a donor reared in the laboratory, the response was remarkably similar regardless of donor species. However, when the donor had been collected from the field, many more genes were differentially expressed. We also found that while the transplant procedure did have some effect on the host transcriptome, this is likely to have had a limited effect on mosquito fitness. Overall, our results highlight the possibility that variation in mosquito microbiome communities is associated with variability in host–microbiome interactions and further demonstrate the utility of the microbiome transplantation technique for investigating host–microbe interactions in mosquitoes.
ORCID iDs
Hegde, Shivanand, Brettell, Laura E., Quek, Shannon, Etebari, Kayvan, Saldaña, Miguel A., Asgari, Sassan, Coon, Kerri L., Heinz, Eva ORCID: https://orcid.org/0000-0003-4413-3756 and Hughes, Grant L.;-
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Item type: Article ID code: 90633 Dates: DateEventFebruary 2024Published8 January 2024Published Online21 December 2023AcceptedSubjects: Science > Microbiology Department: UNSPECIFIED Depositing user: Pure Administrator Date deposited: 23 Sep 2024 10:39 Last modified: 23 Sep 2024 10:39 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/90633