A novel 3D imaging approach for quantification of GLUT4 levels across the intact myocardium
Geiser, Angéline and Currie, Susan and Al-Hasani, Hadi and Chadt, Alexandra and McConnell, Gail and Gould, Gwyn W. (2024) A novel 3D imaging approach for quantification of GLUT4 levels across the intact myocardium. Journal of Cell Science, 137 (20). jcs262146. ISSN 0021-9533 (https://doi.org/10.1242/jcs.262146)
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Abstract
Cellular heterogeneity is a well-accepted feature of tissues, and both transcriptional and metabolic diversity have been revealed by numerous approaches, including optical imaging. However, the high magnification objective lenses needed for high-resolution imaging provides information from only small layers of tissue, which can result in poor cell statistics. There is therefore an unmet need for an imaging modality that can provide detailed molecular and cellular insight within intact tissue samples in 3D. Using GFP-tagged GLUT4 as proof of concept, we present here a novel optical mesoscopy approach that allows precise measurement of the spatial location of GLUT4 within specific anatomical structures across the myocardium in ultrathick sections (5 mm×5 mm×3 mm) of intact mouse heart. We reveal distinct GLUT4 distribution patterns across cardiac walls and highlight specific changes in GLUT4 expression levels in response to high fat diet-feeding, and we identify sex-dependent differences in expression patterns. This method is applicable to any target that can be labelled for light microscopy, and to other complex tissues when organ structure needs to be considered simultaneously with cellular detail.
ORCID iDs
Geiser, Angéline ORCID: https://orcid.org/0000-0002-7108-2440, Currie, Susan ORCID: https://orcid.org/0000-0002-4237-4428, Al-Hasani, Hadi, Chadt, Alexandra, McConnell, Gail ORCID: https://orcid.org/0000-0002-7213-0686 and Gould, Gwyn W. ORCID: https://orcid.org/0000-0001-6571-2875;-
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Item type: Article ID code: 89883 Dates: DateEvent15 October 2024Published5 August 2024Published Online24 June 2024Accepted26 March 2024SubmittedSubjects: Medicine > Biomedical engineering. Electronics. Instrumentation Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Strategic Research Themes > Health and Wellbeing
Strategic Research Themes > Measurement Science and Enabling TechnologiesDepositing user: Pure Administrator Date deposited: 09 Jul 2024 14:44 Last modified: 25 Sep 2024 15:09 URI: https://strathprints.strath.ac.uk/id/eprint/89883