Rational design of a user-friendly aptamer/peptide-based device for the detection of staphylococcus aureus
Ronda, Luca and Tonelli, Alessandro and Sogne, Elisa and Autiero, Ida and Spyrakis, Francesca and Pellegrino, Sara and Abbiati, Giorgio and Maffioli, Elisa and Schulte, Carsten and Piano, Riccardo and Cozzini, Pietro and Mozzarelli, Andrea and Bettati, Stefano and Clerici, Francesca and Milani, Paolo and Lenardi, Cristina and Tedeschi, Gabriella and Gelmi, Maria Luisa (2020) Rational design of a user-friendly aptamer/peptide-based device for the detection of staphylococcus aureus. Sensors (Switzerland), 20 (17). 4977. ISSN 1424-8220 (https://doi.org/10.3390/s20174977)
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Abstract
The urgent need to develop a detection system for Staphylococcus aureus, one of the most common causes of infection, is prompting research towards novel approaches and devices, with a particular focus on point-of-care analysis. Biosensors are promising systems to achieve this aim. We coupled the selectivity and affinity of aptamers, short nucleic acids sequences able to recognize specific epitopes on bacterial surface, immobilized at high density on a nanostructured zirconium dioxide surface, with the rational design of specifically interacting fluorescent peptides to assemble an easy-to-use detection device. We show that the displacement of fluorescent peptides upon the competitive binding of S. aureus to immobilized aptamers can be detected and quantified through fluorescence loss. This approach could be also applied to the detection of other bacterial species once aptamers interacting with specific antigens will be identified, allowing the development of a platform for easy detection of a pathogen without requiring access to a healthcare environment.
ORCID iDs
Ronda, Luca, Tonelli, Alessandro, Sogne, Elisa, Autiero, Ida, Spyrakis, Francesca, Pellegrino, Sara, Abbiati, Giorgio, Maffioli, Elisa, Schulte, Carsten ORCID: https://orcid.org/0000-0002-7554-5342, Piano, Riccardo, Cozzini, Pietro, Mozzarelli, Andrea, Bettati, Stefano, Clerici, Francesca, Milani, Paolo, Lenardi, Cristina, Tedeschi, Gabriella and Gelmi, Maria Luisa;-
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Item type: Article ID code: 89746 Dates: DateEvent2 September 2020Published30 August 2020AcceptedSubjects: Medicine > Biomedical engineering. Electronics. Instrumentation Department: Faculty of Engineering > Biomedical Engineering Depositing user: Pure Administrator Date deposited: 27 Jun 2024 13:00 Last modified: 01 Oct 2024 08:39 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/89746