Scale invariant disordered nanotopography promotes hippocampal neuron development and maturation with involvement of mechanotransductive pathways
Schulte, Carsten and Ripamonti, Maddalena and Maffioli, Elisa and Cappelluti, Martino A. and Nonnis, Simona and Puricelli, Luca and Lamanna, Jacopo and Piazzoni, Claudio and Podestà, Alessandro and Lenardi, Cristina and Tedeschi, Gabriella and Malgaroli, Antonio and Milani, Paolo (2016) Scale invariant disordered nanotopography promotes hippocampal neuron development and maturation with involvement of mechanotransductive pathways. Frontiers in Cellular Neuroscience, 10. 267. ISSN 1662-5102 (https://doi.org/10.3389/fncel.2016.00267)
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Abstract
The identification of biomaterials which promote neuronal maturation up to the generation of integrated neural circuits is fundamental for modern neuroscience. The development of neural circuits arises from complex maturative processes regulated by poorly understood signaling events,often guided by the extracellular matrix (ECM). Here we report that nanostructured zirconia surfaces,produced by supersonic cluster beam deposition of zirconia nanoparticles and characterized by ECM-like nanotopographical features,can direct the maturation of neural networks. Hippocampal neurons cultured on such cluster-assembled surfaces displayed enhanced differentiation paralleled by functional changes. The latter was demonstrated by single-cell electrophysiology showing earlier action potential generation and increased spontaneous postsynaptic currents compared to the neurons grown on the featureless unnaturally flat standard control surfaces. Label-free shotgun proteomics broadly confirmed the functional changes and suggests furthermore a vast impact of the neuron/nanotopography interaction on mechanotransductive machinery components,known to control physiological in vivo ECM-regulated axon guidance and synaptic plasticity. Our results indicate a potential of cluster-assembled zirconia nanotopography exploitable for the creation of efficient neural tissue interfaces and cell culture devices promoting neurogenic events,but also for unveiling mechanotransductive aspects of neuronal development and maturation.
ORCID iDs
Schulte, Carsten ORCID: https://orcid.org/0000-0002-7554-5342, Ripamonti, Maddalena, Maffioli, Elisa, Cappelluti, Martino A., Nonnis, Simona, Puricelli, Luca, Lamanna, Jacopo, Piazzoni, Claudio, Podestà, Alessandro, Lenardi, Cristina, Tedeschi, Gabriella, Malgaroli, Antonio and Milani, Paolo;-
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Item type: Article ID code: 89711 Dates: DateEvent18 November 2016PublishedSubjects: Medicine > Internal medicine > Neuroscience. Biological psychiatry. Neuropsychiatry Department: Faculty of Engineering > Biomedical Engineering Depositing user: Pure Administrator Date deposited: 24 Jun 2024 10:57 Last modified: 03 Oct 2024 09:56 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/89711