Stiffening of DU145 prostate cancer cells driven by actin filaments-microtubule crosstalk conferring resistance to microtubule-targeting drugs
Kubiak, Andrzej and Chighizola, Matteo and Schulte, Carsten and Bryniarska, Natalia and Wesolowska, Julita and Pudelek, Maciej and Lasota, Malgorzata and Ryszawy, Damian and Basta-Kaim, Agnieszka and Laidler, Piotr and Podestà, Alessandro and Lekka, Malgorzata (2021) Stiffening of DU145 prostate cancer cells driven by actin filaments-microtubule crosstalk conferring resistance to microtubule-targeting drugs. Nanoscale, 13 (12). pp. 6212-6226. ISSN 2040-3372 (https://doi.org/10.1039/d0nr06464e)
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Abstract
The crucial role of microtubules in the mitotic-related segregation of chromosomes makes them an excellent target for anticancer microtubule targeting drugs (MTDs) such as vinflunine (VFL), colchicine (COL), and docetaxel (DTX). MTDs affect mitosis by directly perturbing the structural organisation of microtubules. By a direct assessment of the biomechanical properties of prostate cancer DU145 cells exposed to different MTDs using atomic force microscopy, we show that cell stiffening is a response to the application of all the studied MTDs (VFL, COL, DTX). Changes in cellular rigidity are typically attributed to remodelling of the actin filaments in the cytoskeleton. Here, we demonstrate that cell stiffening can be driven by crosstalk between actin filaments and microtubules in MTD-treated cells. Our findings improve the interpretation of biomechanical data obtained for living cells in studies of various physiological and pathological processes.
ORCID iDs
Kubiak, Andrzej, Chighizola, Matteo, Schulte, Carsten ORCID: https://orcid.org/0000-0002-7554-5342, Bryniarska, Natalia, Wesolowska, Julita, Pudelek, Maciej, Lasota, Malgorzata, Ryszawy, Damian, Basta-Kaim, Agnieszka, Laidler, Piotr, Podestà, Alessandro and Lekka, Malgorzata;-
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Item type: Article ID code: 89700 Dates: DateEvent28 March 2021Published26 February 2021Published Online26 February 2021AcceptedSubjects: Technology > Engineering (General). Civil engineering (General) > Bioengineering
Medicine > Biomedical engineering. Electronics. Instrumentation
Medicine > Internal medicine > Neoplasms. Tumors. Oncology (including Cancer)
Medicine > Therapeutics. PharmacologyDepartment: Faculty of Engineering > Biomedical Engineering Depositing user: Pure Administrator Date deposited: 21 Jun 2024 14:57 Last modified: 03 Oct 2024 10:21 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/89700