Kindlin-1 is a phosphoprotein involved in regulation of polarity, proliferation, and motility of epidermal keratinocytes
Herz, Corinna and Aumailley, Monique and Schulte, Carsten and Schlötzer-Schrehardt, Ursula and Bruckner-Tuderman, Leena and Has, Cristina (2006) Kindlin-1 is a phosphoprotein involved in regulation of polarity, proliferation, and motility of epidermal keratinocytes. Journal of Biological Chemistry, 281 (47). pp. 36082-36090. ISSN 1083-351X (https://doi.org/10.1074/jbc.M606259200)
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Abstract
A novel family of focal adhesion proteins, the kindlins, is involved in attachment of the actin cytoskeleton to the plasma membrane and in integrin-mediated cellular processes. Deficiency of kindlin-1, as a result of loss-of-function mutations in the KIND1 gene, causes Kindler syndrome, an autosomal recessive genodermatosis characterized by skin blistering, progressive skin atrophy, photosensitivity and, occasionally, carcinogenesis. Here we characterized authentic and recombinantly expressed kindlin-1 and show that it is localized in basal epidermal keratinocytes in a polar fashion, close to the cell surface facing the basement membrane, in the areas between the hemidesmosomes. We identified two forms of kindlin-1 in keratinocytes, with apparent molecular masses of 78 and 74 kDa, corresponding to phosphorylated and desphosphorylated forms of the protein. In kindlin-1-deficient skin, basal keratinocytes show multiple abnormalities: cell polarity is lost, proliferation is strongly reduced, and several cells undergo apoptosis. In vitro, deficiency of kindlin-1 in keratinocytes leads to strongly reduced cell proliferation, decreased adhesion, undirected motility, and intense protrusion activity of the plasma membrane. Taken together, these results show that kindlin-1 plays a role in keratinocyte adhesion, polarization, proliferation, and migration. It is involved in organization and anchorage of the actin cytoskeleton to integrin-associated signaling platforms.
ORCID iDs
Herz, Corinna, Aumailley, Monique, Schulte, Carsten ORCID: https://orcid.org/0000-0002-7554-5342, Schlötzer-Schrehardt, Ursula, Bruckner-Tuderman, Leena and Has, Cristina;-
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Item type: Article ID code: 89698 Dates: DateEvent24 November 2006Published1 October 2006Published OnlineSubjects: Medicine > Biomedical engineering. Electronics. Instrumentation
Technology > Engineering (General). Civil engineering (General) > Bioengineering
Science > MicrobiologyDepartment: Faculty of Engineering > Biomedical Engineering Depositing user: Pure Administrator Date deposited: 21 Jun 2024 14:23 Last modified: 03 Oct 2024 09:20 URI: https://strathprints.strath.ac.uk/id/eprint/89698