Force sensing on cells and tissues by atomic force microscopy
Holuigue, Hatice and Lorenc, Ewelina and Chighizola, Matteo and Schulte, Carsten and Varinelli, Luca and Deraco, Marcello and Guaglio, Marcello and Gariboldi, Manuela and Podestà, Alessandro (2022) Force sensing on cells and tissues by atomic force microscopy. Sensors, 22 (6). 2197. ISSN 1424-8220 (https://doi.org/10.3390/s22062197)
Preview |
Text.
Filename: Holuigue-etal-Sensors-2022-Force-sensing-on-cells-and-tissues_by-atomic-force-microscopy.pdf
Final Published Version License: Download (25MB)| Preview |
Abstract
Biosensors are aimed at detecting tiny physical and chemical stimuli in biological systems. Physical forces are ubiquitous, being implied in all cellular processes, including cell adhesion, migration, and differentiation. Given the strong interplay between cells and their microenvironment, the extracellular matrix (ECM) and the structural and mechanical properties of the ECM play an important role in the transmission of external stimuli to single cells within the tissue. Vice versa, cells themselves also use self-generated forces to probe the biophysical properties of the ECM. ECM mechanics influence cell fate, regulate tissue development, and show peculiar features in health and disease conditions of living organisms. Force sensing in biological systems is therefore crucial to dissecting and understanding complex biological processes, such as mechanotransduction. Atomic Force Microscopy (AFM), which can both sense and apply forces at the nanoscale, with sub-nanonewton sensitivity, represents an enabling technology and a crucial experimental tool in biophysics and mechanobiology. In this work, we report on the application of AFM to the study of biomechanical fingerprints of different components of biological systems, such as the ECM, the whole cell, and cellular components, such as the nucleus, lamellipodia and the glycocalyx. We show that physical observables such as the (spatially resolved) Young’s Modulus (YM) of elasticity of ECMs or cells, and the effective thickness and stiffness of the glycocalyx, can be quantitatively characterized by AFM. Their modification can be correlated to changes in the microenvironment, physio-pathological conditions, or gene regulation.
-
-
Item type: Article ID code: 89694 Dates: DateEvent11 March 2022Published11 March 2022Published Online9 March 2022AcceptedSubjects: Medicine > Biomedical engineering. Electronics. Instrumentation
Technology > Engineering (General). Civil engineering (General) > Bioengineering
Science > Chemistry > Analytical chemistryDepartment: Faculty of Engineering > Biomedical Engineering Depositing user: Pure Administrator Date deposited: 21 Jun 2024 13:32 Last modified: 11 Aug 2024 01:38 URI: https://strathprints.strath.ac.uk/id/eprint/89694