Nanotopography and microconfinement impact on primary hippocampal astrocyte morphology, cytoskeleton and spontaneous calcium wave signalling
Previdi, Anita and Borghi, Francesca and Profumo, Filippo and Schulte, Carsten and Piazzoni, Claudio and Lamanna, Jacopo and Racchetti, Gabriella and Malgaroli, Antonio and Milani, Paolo (2023) Nanotopography and microconfinement impact on primary hippocampal astrocyte morphology, cytoskeleton and spontaneous calcium wave signalling. Cells, 12 (2). 293. ISSN 2073-4409 (https://doi.org/10.3390/cells12020293)
Preview |
Text.
Filename: Previdi-etal-Cells-2023-Nanotopography-and-microconfinement-impact-on-primary-hippocampal-astrocyte-morphology.pdf
Final Published Version License: Download (4MB)| Preview |
Abstract
Astrocytes' organisation affects the functioning and the fine morphology of the brain, both in physiological and pathological contexts. Although many aspects of their role have been characterised, their complex functions remain, to a certain extent, unclear with respect to their contribution to brain cell communication. Here, we studied the effects of nanotopography and microconfinement on primary hippocampal rat astrocytes. For this purpose, we fabricated nanostructured zirconia surfaces as homogenous substrates and as micrometric patterns, the latter produced by a combination of an additive nanofabrication and micropatterning technique. These engineered substrates reproduce both nanotopographical features and microscale geometries that astrocytes encounter in their natural environment, such as basement membrane topography, as well as blood vessels and axonal fibre topology. The impact of restrictive adhesion manifests in the modulation of several cellular properties of single cells (morphological and actin cytoskeletal changes) and the network organisation and functioning. Calcium wave signalling was observed only in astrocytes grown in confined geometries, with an activity enhancement in cells forming elongated agglomerates with dimensions typical of blood vessels or axon fibres. Our results suggest that calcium oscillation and wave propagation are closely related to astrocytic morphology and actin cytoskeleton organisation.
ORCID iDs
Previdi, Anita, Borghi, Francesca, Profumo, Filippo, Schulte, Carsten ORCID: https://orcid.org/0000-0002-7554-5342, Piazzoni, Claudio, Lamanna, Jacopo, Racchetti, Gabriella, Malgaroli, Antonio and Milani, Paolo;-
-
Item type: Article ID code: 89692 Dates: DateEvent12 January 2023Published12 January 2023Published Online10 January 2023AcceptedSubjects: Medicine > Biomedical engineering. Electronics. Instrumentation
Science > MicrobiologyDepartment: Faculty of Engineering > Biomedical Engineering Depositing user: Pure Administrator Date deposited: 21 Jun 2024 13:24 Last modified: 03 Oct 2024 09:34 URI: https://strathprints.strath.ac.uk/id/eprint/89692