Microbial reduction of prebagged human plasma using 405nm light and its effects on coagulation factors
Stewart, Caitlin F. and McGoldrick, Preston and Anderson, John G. and MacGregor, Scott J. and Atreya, Chintamani D. and Maclean, Michelle (2024) Microbial reduction of prebagged human plasma using 405nm light and its effects on coagulation factors. AMB Express, 14 (1). 66. ISSN 2191-0855 (https://doi.org/10.1186/s13568-024-01725-0)
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Abstract
Bacterial contamination is the most prevalent infectious complication of blood transfusion in the developed world. To mitigate this, several ultraviolet light-based pathogen reduction technologies (PRTs), some of which require photo-chemicals, have been developed to minimize infection transmission. Relative to UV light, visible 405-nm light is safer and has shown potential to be developed as a PRT for the in situ treatment of ex vivo human plasma and platelet concentrates, without the need for photo-chemicals. This study investigates the effect of 405-nm light on human plasma, with focus on the compatibility of antimicrobial light doses with essential plasma clotting factors. To determine an effective antimicrobial dose that is compatible with plasma, prebagged human plasma (up to 300 mL) was seeded with common microbial contaminants and treated with increasing doses of 405-nm light (16 mW cm−2; ≤ 403 J cm−2). Post-exposure plasma protein integrity was investigated using an AOPP assay, in vitro coagulation tests, and ELISA-based measurement of fibrinogen and Protein S. Microbial contamination in 300 mL prebagged human plasma was significantly reduced (P ≤ 0.05) after exposure to ≤ 288 J cm−2, with microbial loads reduced by > 96.2%. This dose did not significantly affect the plasma protein quality parameters tested (P > 0.05). Increased doses (≥ 345 J cm−2) resulted in a 4.3% increase in clot times with no statistically significant change in protein activity or levels. Overall, this study has demonstrated that the effective microbicidal 405 light dose shows little to no negative effect on plasma quality.
ORCID iDs
Stewart, Caitlin F., McGoldrick, Preston, Anderson, John G. ORCID: https://orcid.org/0000-0003-4151-1619, MacGregor, Scott J. ORCID: https://orcid.org/0000-0002-0808-585X, Atreya, Chintamani D. and Maclean, Michelle ORCID: https://orcid.org/0000-0001-5750-0397;-
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Item type: Article ID code: 89497 Dates: DateEvent6 June 2024Published27 May 2024AcceptedSubjects: Science > Microbiology Department: Faculty of Engineering > Electronic and Electrical Engineering Depositing user: Pure Administrator Date deposited: 07 Jun 2024 09:05 Last modified: 25 Sep 2024 14:55 URI: https://strathprints.strath.ac.uk/id/eprint/89497