In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation

Arnold, S. Alison and Crowley, J. and Woods, N. and Harvey, L.M. and McNeil, B. (2003) In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation. Biotechnology and Bioengineering, 84 (1). pp. 13-19. ISSN 0006-3592

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Abstract

The use of in-situ near infrared spectroscopy (NIRS) as a tool for monitoring four key analytes in a CHO-K1 animal cell culture was investigated. Previous work using on-line NIRS to monitor bioprocesses has involved its application ex-situ where the analyzer is physically outside the fermentor, or to microbial bioprocesses. This novel application of NIRS to monitor analytes within an animal cell culture using a steam sterilizable in-situ fiber optic probe is very important for furthering the use of NIRS within the bioprocessing industry. The method of calibration used to develop the models involved the use of large data sets so that all likely variation in stoichiometry was incorporated within the models. Successful models for glucose, lactate, glutamine, and ammonia were built with Standard Error of Predictions (SEP's) of 0.072 (g/L), 0.0144 (g/L), 0.308 (mM), and 0.036 (mM), respectively of the total concentration range.

Creators(s): Arnold, S. Alison, Crowley, J., Woods, N., Harvey, L.M. and McNeil, B.;
Item type:Article
ID code:8377
Keywords:CHO cells, in-situ, NIRS, infrared spectroscopy, cell cultivation, cells, biosciences, Physiology, Biology, Therapeutics. Pharmacology, Applied Microbiology and Biotechnology, Bioengineering, Biotechnology
Subjects:Science > Physiology
Science > Natural history > Biology
Medicine > Therapeutics. Pharmacology
Department:Unknown Department
Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences
Depositing user: Strathprints Administrator
Date deposited:04 Aug 2009 11:01
Last modified:20 Jan 2021 17:46
URI:https://strathprints.strath.ac.uk/id/eprint/8377
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