Facile impedimetric analysis of neuronal exosome markers in Parkinson's disease diagnostics

Fu, Ying and Jiang, Cheng and Tofaris, George K. and Davis, Jason J. (2020) Facile impedimetric analysis of neuronal exosome markers in Parkinson's disease diagnostics. Analytical Chemistry, 92 (20). 13647–13651. ISSN 0003-2700 (https://doi.org/10.1021/acs.analchem.0c03092)

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Abstract

The egress of α-synuclein in neuronally derived exosomes predates the clinical presentation of Parkinson’s disease (PD), offering a means of developing a predictive or prognostic test. Here, we report the reagentless impedimetric assay of two internal exosome markers (α-synuclein and syntenin-1) from neuronal exosomes. Exosomes were efficiently extracted from patient sera using anti-L1CAM conjugated zwitterionic polymer-modified magnetic beads prior to lysis and analyzed by electrochemical impedance spectroscopy. The quantification of α-synuclein level across 40 clinical samples resolved statistically significant differences between PD patients and healthy controls (HC).

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