Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor

Chawjiraphan, Wireeya and Apiwat, Chayachon and Segkhoonthod, Khoonsake and Treerattrakoon, Kiatnida and Pinpradup, Preedee and Sathirapongsasuti, Nuankanya and Pongprayoon, Prapasiri and Luksirikul, Patraporn and Isarankura-Na-Ayudhya, Patcharee and Japrung, Deanpen (2020) Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor. MethodsX, 7. 101114. ISSN 2215-0161

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    Abstract

    A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample. The limit of detection of our developed platform is 0.05 µg.mL−1. The detection range of our aptasensor is 0.1–600 µg.mL−1