Cilia density and flow velocity affect alignment of motile cilia from brain cells
Pellicciotta, Nicola and Das, Debasish and Kotar, Jurij and Faucourt, Marion and Spassky, Nathalie and Lauga, Eric and Cicuta, Pietro (2020) Cilia density and flow velocity affect alignment of motile cilia from brain cells. Journal of Experimental Biology, 223 (24). jeb229310. ISSN 0022-0949 (https://doi.org/10.1242/jeb.229310)
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Abstract
In many organs, thousands of microscopic ‘motile cilia’ beat in a coordinated fashion generating fluid flow. Physiologically, these flows are important in both development and homeostasis of ciliated tissues. Combining experiments and simulations, we studied how cilia from brain tissue align their beating direction. We subjected cilia to a broad range of shear stresses, similar to the fluid flow that cilia themselves generate, in a microfluidic setup. In contrast to previous studies, we found that cilia from mouse ependyma respond and align to these physiological shear stress at all maturation stages. Cilia align more easily earlier in maturation, and we correlated this property with the increase in multiciliated cell density during maturation. Our numerical simulations show that cilia in densely packed clusters are hydrodynamically screened from the external flow, in agreement with our experimental observation. Cilia carpets create a hydrodynamic screening that reduces the susceptibility of individual cilia to external flows.
ORCID iDs
Pellicciotta, Nicola, Das, Debasish ORCID: https://orcid.org/0000-0003-2365-4720, Kotar, Jurij, Faucourt, Marion, Spassky, Nathalie, Lauga, Eric and Cicuta, Pietro;-
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Item type: Article ID code: 74993 Dates: DateEvent29 December 2020Published29 October 2020AcceptedSubjects: Social Sciences > Statistics
Medicine > Internal medicine > Neuroscience. Biological psychiatry. NeuropsychiatryDepartment: Faculty of Science > Mathematics and Statistics Depositing user: Pure Administrator Date deposited: 07 Jan 2021 12:59 Last modified: 17 Sep 2024 22:17 URI: https://strathprints.strath.ac.uk/id/eprint/74993