Cryptic or silent? The known unknowns, unknown knowns, and unknown unknowns of secondary metabolism
Hoskisson, Paul A. and Seipke, Ryan F. (2020) Cryptic or silent? The known unknowns, unknown knowns, and unknown unknowns of secondary metabolism. mBio, 11 (5). e02642-20. ISSN 2161-2129 (https://doi.org/10.1128/mBio.02642-20)
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Abstract
Microbial natural products, particularly those produced by filamentous Actinobacteria, underpin the majority of clinically used antibiotics. Unfortunately, only a few new antibiotic classes have been discovered since the 1970s, which has exacerbated fears of a postapocalyptic world in which antibiotics have lost their utility. Excitingly, the genome sequencing revolution painted an entirely new picture, one in which an average strain of filamentous Actinobacteria harbors 20 to 50 natural product biosynthetic pathways but expresses very few of these under laboratory conditions. Development of methodology to access this “hidden” biochemical diversity has the potential to usher in a second Golden Era of antibiotic discovery. The proliferation of genomic data has led to inconsistent use of “cryptic” and “silent” when referring to biosynthetic gene clusters identified by bioinformatic analysis. In this Perspective, we discuss this issue and propose to formalize the use of this terminology.
ORCID iDs
Hoskisson, Paul A. ORCID: https://orcid.org/0000-0003-4332-1640 and Seipke, Ryan F.;-
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Item type: Article ID code: 74696 Dates: DateEvent20 October 2020Published1 September 2020AcceptedSubjects: Medicine > Pharmacy and materia medica Department: Faculty of Science > Strathclyde Institute of Pharmacy and Biomedical Sciences Depositing user: Pure Administrator Date deposited: 25 Nov 2020 15:47 Last modified: 24 Nov 2024 01:21 Related URLs: URI: https://strathprints.strath.ac.uk/id/eprint/74696